Review



cell transfection primary human epidermal melanocytes  (PromoCell)


Bioz Verified Symbol PromoCell is a verified supplier
Bioz Manufacturer Symbol PromoCell manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    PromoCell cell transfection primary human epidermal melanocytes
    Figure 2. Upregulation of miR-493 suppressed human melanoma cell proliferation. (a) Validation of miR-493 expression levels after <t>transfection</t> by PCR analysis. (b) MTT assays revealed that inhibition of miR-493 suppressed growth of A375 cell line. (c) Upregulation of miR-493 inhibited the anchorage-independent growth of A375 cells. Representative micrographs (left) and quantification of colonies that were >0.1 mm (right). (d) Representative micrographs (left) and quantification (right) of the BrdU incorporation assay in A375 cells. (e) Flow cytometric analysis of the indicated A375 cells transfected with NC or miR-493. Each bar represents the mean of three independent experiments (*p < 0.05).
    Cell Transfection Primary Human Epidermal Melanocytes, supplied by PromoCell, used in various techniques. Bioz Stars score: 94/100, based on 110 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+transfection+primary+human+epidermal+melanocytes/pm28475006-26-3-10?v=PromoCell
    Average 94 stars, based on 110 article reviews
    cell transfection primary human epidermal melanocytes - by Bioz Stars, 2026-07
    94/100 stars

    Images

    1) Product Images from "Downregulation of miR-493 promoted melanoma proliferation by suppressing IRS4 expression."

    Article Title: Downregulation of miR-493 promoted melanoma proliferation by suppressing IRS4 expression.

    Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

    doi: 10.1177/1010428317701640

    Figure 2. Upregulation of miR-493 suppressed human melanoma cell proliferation. (a) Validation of miR-493 expression levels after transfection by PCR analysis. (b) MTT assays revealed that inhibition of miR-493 suppressed growth of A375 cell line. (c) Upregulation of miR-493 inhibited the anchorage-independent growth of A375 cells. Representative micrographs (left) and quantification of colonies that were >0.1 mm (right). (d) Representative micrographs (left) and quantification (right) of the BrdU incorporation assay in A375 cells. (e) Flow cytometric analysis of the indicated A375 cells transfected with NC or miR-493. Each bar represents the mean of three independent experiments (*p < 0.05).
    Figure Legend Snippet: Figure 2. Upregulation of miR-493 suppressed human melanoma cell proliferation. (a) Validation of miR-493 expression levels after transfection by PCR analysis. (b) MTT assays revealed that inhibition of miR-493 suppressed growth of A375 cell line. (c) Upregulation of miR-493 inhibited the anchorage-independent growth of A375 cells. Representative micrographs (left) and quantification of colonies that were >0.1 mm (right). (d) Representative micrographs (left) and quantification (right) of the BrdU incorporation assay in A375 cells. (e) Flow cytometric analysis of the indicated A375 cells transfected with NC or miR-493. Each bar represents the mean of three independent experiments (*p < 0.05).

    Techniques Used: Biomarker Discovery, Expressing, Transfection, Inhibition, BrdU Incorporation Assay

    Figure 3. Inhibition of miR-493 promoted human melanoma cell proliferation. (a) Validation of miR-493 expression levels after transfection by PCR analysis. (b) MTT assays revealed that miR-493-in promoted A375 cell growth. (c) Inhibition of miR-493 inhibited the anchorage-independent growth of A375 cells. Representative micrographs (left) and quantification of colonies that were >0.1 mm (right). (d) Representative micrographs (left) and quantification (right) of the BrdU incorporation assays in A375 cells. (e) Flow cytometric analysis of the indicated A375 cells transfected with NC or miR-493-in. Each bar represents the mean of three independent experiments (*p < 0.05).
    Figure Legend Snippet: Figure 3. Inhibition of miR-493 promoted human melanoma cell proliferation. (a) Validation of miR-493 expression levels after transfection by PCR analysis. (b) MTT assays revealed that miR-493-in promoted A375 cell growth. (c) Inhibition of miR-493 inhibited the anchorage-independent growth of A375 cells. Representative micrographs (left) and quantification of colonies that were >0.1 mm (right). (d) Representative micrographs (left) and quantification (right) of the BrdU incorporation assays in A375 cells. (e) Flow cytometric analysis of the indicated A375 cells transfected with NC or miR-493-in. Each bar represents the mean of three independent experiments (*p < 0.05).

    Techniques Used: Inhibition, Biomarker Discovery, Expressing, Transfection, BrdU Incorporation Assay

    Figure 5. IRS4 downregulation counteracted the proliferation promotion by miR-493-in. (a) Western blot analysis verified that silencing of IRS4 effectively decreased the expression of IRS4 in miR-493-in-transfected A375 cells. (b) MiR-493-in-transfected A375 cells after transfection with IRS4 siRNAs promoted the anchorage-independent growth. Representative quantification of colonies that were >0.1 mm. (c) Representative micrographs (left) and quantification (right) of the BrdU incorporation assays. Each bar represents the mean of three independent experiments (*p < 0.05).
    Figure Legend Snippet: Figure 5. IRS4 downregulation counteracted the proliferation promotion by miR-493-in. (a) Western blot analysis verified that silencing of IRS4 effectively decreased the expression of IRS4 in miR-493-in-transfected A375 cells. (b) MiR-493-in-transfected A375 cells after transfection with IRS4 siRNAs promoted the anchorage-independent growth. Representative quantification of colonies that were >0.1 mm. (c) Representative micrographs (left) and quantification (right) of the BrdU incorporation assays. Each bar represents the mean of three independent experiments (*p < 0.05).

    Techniques Used: Western Blot, Expressing, Transfection, BrdU Incorporation Assay



    Similar Products

    cell transfection primary human epidermal melanocytes  (PromoCell)
    94
    PromoCell cell transfection primary human epidermal melanocytes
    Figure 2. Upregulation of miR-493 suppressed human melanoma cell proliferation. (a) Validation of miR-493 expression levels after <t>transfection</t> by PCR analysis. (b) MTT assays revealed that inhibition of miR-493 suppressed growth of A375 cell line. (c) Upregulation of miR-493 inhibited the anchorage-independent growth of A375 cells. Representative micrographs (left) and quantification of colonies that were >0.1 mm (right). (d) Representative micrographs (left) and quantification (right) of the BrdU incorporation assay in A375 cells. (e) Flow cytometric analysis of the indicated A375 cells transfected with NC or miR-493. Each bar represents the mean of three independent experiments (*p < 0.05).
    Cell Transfection Primary Human Epidermal Melanocytes, supplied by PromoCell, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+transfection+primary+human+epidermal+melanocytes/pm28475006-26-3-10?v=PromoCell
    Average 94 stars, based on 1 article reviews
    cell transfection primary human epidermal melanocytes - by Bioz Stars, 2026-07
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Figure 2. Upregulation of miR-493 suppressed human melanoma cell proliferation. (a) Validation of miR-493 expression levels after transfection by PCR analysis. (b) MTT assays revealed that inhibition of miR-493 suppressed growth of A375 cell line. (c) Upregulation of miR-493 inhibited the anchorage-independent growth of A375 cells. Representative micrographs (left) and quantification of colonies that were >0.1 mm (right). (d) Representative micrographs (left) and quantification (right) of the BrdU incorporation assay in A375 cells. (e) Flow cytometric analysis of the indicated A375 cells transfected with NC or miR-493. Each bar represents the mean of three independent experiments (*p < 0.05).

    Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

    Article Title: Downregulation of miR-493 promoted melanoma proliferation by suppressing IRS4 expression.

    doi: 10.1177/1010428317701640

    Figure Lengend Snippet: Figure 2. Upregulation of miR-493 suppressed human melanoma cell proliferation. (a) Validation of miR-493 expression levels after transfection by PCR analysis. (b) MTT assays revealed that inhibition of miR-493 suppressed growth of A375 cell line. (c) Upregulation of miR-493 inhibited the anchorage-independent growth of A375 cells. Representative micrographs (left) and quantification of colonies that were >0.1 mm (right). (d) Representative micrographs (left) and quantification (right) of the BrdU incorporation assay in A375 cells. (e) Flow cytometric analysis of the indicated A375 cells transfected with NC or miR-493. Each bar represents the mean of three independent experiments (*p < 0.05).

    Article Snippet: Cell culture and cell transfection Primary human epidermal melanocytes (PEM; PromoCell, Germany) were purchased from ScienCell (USA) and were grown in serum- and phorbol myristate acetate (PMA)-free melanocyte growth medium M2 (PromoCell, Germany).

    Techniques: Biomarker Discovery, Expressing, Transfection, Inhibition, BrdU Incorporation Assay

    Figure 3. Inhibition of miR-493 promoted human melanoma cell proliferation. (a) Validation of miR-493 expression levels after transfection by PCR analysis. (b) MTT assays revealed that miR-493-in promoted A375 cell growth. (c) Inhibition of miR-493 inhibited the anchorage-independent growth of A375 cells. Representative micrographs (left) and quantification of colonies that were >0.1 mm (right). (d) Representative micrographs (left) and quantification (right) of the BrdU incorporation assays in A375 cells. (e) Flow cytometric analysis of the indicated A375 cells transfected with NC or miR-493-in. Each bar represents the mean of three independent experiments (*p < 0.05).

    Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

    Article Title: Downregulation of miR-493 promoted melanoma proliferation by suppressing IRS4 expression.

    doi: 10.1177/1010428317701640

    Figure Lengend Snippet: Figure 3. Inhibition of miR-493 promoted human melanoma cell proliferation. (a) Validation of miR-493 expression levels after transfection by PCR analysis. (b) MTT assays revealed that miR-493-in promoted A375 cell growth. (c) Inhibition of miR-493 inhibited the anchorage-independent growth of A375 cells. Representative micrographs (left) and quantification of colonies that were >0.1 mm (right). (d) Representative micrographs (left) and quantification (right) of the BrdU incorporation assays in A375 cells. (e) Flow cytometric analysis of the indicated A375 cells transfected with NC or miR-493-in. Each bar represents the mean of three independent experiments (*p < 0.05).

    Article Snippet: Cell culture and cell transfection Primary human epidermal melanocytes (PEM; PromoCell, Germany) were purchased from ScienCell (USA) and were grown in serum- and phorbol myristate acetate (PMA)-free melanocyte growth medium M2 (PromoCell, Germany).

    Techniques: Inhibition, Biomarker Discovery, Expressing, Transfection, BrdU Incorporation Assay

    Figure 5. IRS4 downregulation counteracted the proliferation promotion by miR-493-in. (a) Western blot analysis verified that silencing of IRS4 effectively decreased the expression of IRS4 in miR-493-in-transfected A375 cells. (b) MiR-493-in-transfected A375 cells after transfection with IRS4 siRNAs promoted the anchorage-independent growth. Representative quantification of colonies that were >0.1 mm. (c) Representative micrographs (left) and quantification (right) of the BrdU incorporation assays. Each bar represents the mean of three independent experiments (*p < 0.05).

    Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

    Article Title: Downregulation of miR-493 promoted melanoma proliferation by suppressing IRS4 expression.

    doi: 10.1177/1010428317701640

    Figure Lengend Snippet: Figure 5. IRS4 downregulation counteracted the proliferation promotion by miR-493-in. (a) Western blot analysis verified that silencing of IRS4 effectively decreased the expression of IRS4 in miR-493-in-transfected A375 cells. (b) MiR-493-in-transfected A375 cells after transfection with IRS4 siRNAs promoted the anchorage-independent growth. Representative quantification of colonies that were >0.1 mm. (c) Representative micrographs (left) and quantification (right) of the BrdU incorporation assays. Each bar represents the mean of three independent experiments (*p < 0.05).

    Article Snippet: Cell culture and cell transfection Primary human epidermal melanocytes (PEM; PromoCell, Germany) were purchased from ScienCell (USA) and were grown in serum- and phorbol myristate acetate (PMA)-free melanocyte growth medium M2 (PromoCell, Germany).

    Techniques: Western Blot, Expressing, Transfection, BrdU Incorporation Assay